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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0395Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
100IU/L	2.069	2.102	2.021
	2.135
50IU/L	1.155	1.115	1.034
	1.075
25IU/L	0.567	0.551	0.47
	0.534
12.5IU/L	0.295	0.283	0.202
	0.27
6.25IU/L	0.161	0.151	0.07
	0.141
0IU/L	0.075	0.081	0
	0.087

We measured random samples of E0395Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	28	1	3.5
Intra-assay	2	18	44.3	1.6	3.7
Intra-assay	3	18	79.9	2.1	2.6

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