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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1483Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.279	2.26	2.231
	2.241
8ng/ml	1.43	1.401	1.373
	1.373
4ng/ml	0.583	0.57	0.541
	0.556
2ng/ml	0.331	0.318	0.29
	0.306
1ng/ml	0.188	0.16	0.132
	0.132
0ng/ml	0.015	0.028	0
	0.041

We measured random samples of E1483Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	8.4	0.3	3
Intra-assay	2	18	3.4	0.1	4.2
Intra-assay	3	18	6.1	0.2	3.4

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