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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1413Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/ml	2.112	2.059	2.03
	2.006
800ng/ml	1.103	1.047	1.018
	0.992
400ng/ml	0.422	0.45	0.421
	0.479
200ng/ml	0.172	0.176	0.147
	0.179
100ng/ml	0.081	0.118	0.088
	0.155
0ng/ml	0.029	0.029	0
	0.03

We measured random samples of E1413Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	732.8	25	3.4
Intra-assay	2	18	947.8	24.7	2.6
Intra-assay	3	18	517.3	23.3	4.5

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