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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E3700Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.96	1.998	1.916
	2.036
12ng/ml	1.081	1.039	0.956
	0.996
6ng/ml	0.594	0.578	0.495
	0.561
3ng/ml	0.292	0.278	0.195
	0.264
1.5ng/ml	0.165	0.156	0.074
	0.148
0ng/ml	0.087	0.082	0
	0.078

We measured random samples of E3700Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	13.3	0.8	6.2
Intra-assay	2	18	17.6	0.5	2.9
Intra-assay	3	18	5	0.2	4.4

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