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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1146Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.024	1.958	1.92
	1.892
40ng/ml	1.089	0.998	0.96
	0.907
20ng/ml	0.478	0.502	0.464
	0.526
10ng/ml	0.188	0.228	0.189
	0.267
5ng/ml	0.129	0.103	0.065
	0.078
0ng/ml	0.036	0.038	0
	0.041

We measured random samples of E1146Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	25.2	1.4	5.7
Intra-assay	2	18	65.6	1.7	2.6
Intra-assay	3	18	31.6	1.4	4.3

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