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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0221Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.251	2.285	2.244
	2.319
1200ng/L	1.356	1.281	1.24
	1.207
600ng/L	0.79	0.824	0.783
	0.859
300ng/L	0.486	0.507	0.466
	0.529
150ng/L	0.285	0.304	0.263
	0.324
0ng/L	0.043	0.041	0
	0.039

We measured random samples of E0221Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	806.1	55.9	6.9
Intra-assay	2	18	1101.3	40.9	3.7
Intra-assay	3	18	337.6	25.9	7.7

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