Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
UniProt accession
MASS（Da）
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0616Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 400ug/ml | 2.023 | 1.965 | 1.88 |
| 1.906 | |||
| 200ug/ml | 1.146 | 1.17 | 1.086 |
| 1.195 | |||
| 100ug/ml | 0.581 | 0.569 | 0.484 |
| 0.557 | |||
| 50ug/ml | 0.284 | 0.273 | 0.188 |
| 0.261 | |||
| 25ug/ml | 0.16 | 0.17 | 0.085 |
| 0.18 | |||
| 0ug/ml | 0.088 | 0.085 | 0 |
| 0.081 |
Precision
We measured random samples of E0616Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 236.4 | 8.5 | 3.6 |
| Intra-assay | 2 | 18 | 75.7 | 3.3 | 4.4 |
| Intra-assay | 3 | 18 | 141.9 | 6.1 | 4.3 |
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