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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1455Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.175	2.189	2.12
	2.203
60ng/ml	1.175	1.144	1.075
	1.113
30ng/ml	0.568	0.582	0.513
	0.596
15ng/ml	0.319	0.336	0.267
	0.352
7.5ng/ml	0.167	0.163	0.094
	0.159
0ng/ml	0.064	0.069	0
	0.073

We measured random samples of E1455Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	63.8	3.3	5.2
Intra-assay	2	18	82	2	2.5
Intra-assay	3	18	34.4	1.5	4.4

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