Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0110GO are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/L | 2.167 | 2.121 | 2.082 |
| 2.075 | |||
| 240ng/L | 1.177 | 1.24 | 1.201 |
| 1.303 | |||
| 120ng/L | 0.815 | 0.77 | 0.732 |
| 0.726 | |||
| 60ng/L | 0.469 | 0.452 | 0.413 |
| 0.435 | |||
| 30ng/L | 0.177 | 0.216 | 0.177 |
| 0.256 | |||
| 0ng/L | 0.037 | 0.039 | 0 |
| 0.041 |
Precision
We measured random samples of E0110GO within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 188.7 | 12.3 | 6.5 |
| Intra-assay | 2 | 18 | 261.6 | 7.2 | 2.8 |
| Intra-assay | 3 | 18 | 82.4 | 5 | 6.1 |
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