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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0465Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
96ng/ml	2.142	2.137	2.094
	2.133
48ng/ml	1.625	1.667	1.624
	1.708
24ng/ml	1.18	1.123	1.08
	1.066
12ng/ml	0.669	0.666	0.623
	0.663
6ng/ml	0.427	0.422	0.38
	0.418
0ng/ml	0.046	0.043	0
	0.039

We measured random samples of E0465Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	54.2	3.1	5.8
Intra-assay	2	18	13.7	0.7	5.3
Intra-assay	3	18	5.9	0.4	6.1

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