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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E2013Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200nmol/ml	1.98	2.019	1.942
	2.058
1600nmol/ml	1.16	1.185	1.108
	1.21
800nmol/ml	0.613	0.597	0.52
	0.58
400nmol/ml	0.296	0.303	0.226
	0.31
200nmol/ml	0.159	0.15	0.073
	0.141
0nmol/ml	0.071	0.077	0
	0.083

We measured random samples of E2013Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	2014.9	57.2	2.8
Intra-assay	2	18	781.1	33.8	4.3
Intra-assay	3	18	1123.2	45.4	4

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