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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0013Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	1.975	2.042	1.973
	2.108
3.2ng/ml	1.078	1.114	1.045
	1.15
1.6ng/ml	0.588	0.57	0.501
	0.552
0.8ng/ml	0.315	0.331	0.262
	0.347
0.4ng/ml	0.178	0.182	0.113
	0.185
0ng/ml	0.072	0.069	0
	0.065

We measured random samples of E0013Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	4.9	0.1	2.2
Intra-assay	2	18	1.8	0.1	5.6
Intra-assay	3	18	2.4	0.1	4.4

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