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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0023Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.037	2.055	1.979
	2.074
60ng/ml	1.052	1.037	0.96
	1.021
30ng/ml	0.589	0.602	0.525
	0.614
15ng/ml	0.325	0.345	0.268
	0.364
7.5ng/ml	0.164	0.16	0.083
	0.155
0ng/ml	0.071	0.077	0
	0.082

We measured random samples of E0023Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	106.9	3.4	3.2
Intra-assay	2	18	46	2	4.5
Intra-assay	3	18	34.6	2.3	6.7

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