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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E7195Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.258	2.248	2.224
	2.237
8ng/ml	1.42	1.314	1.29
	1.208
4ng/ml	0.715	0.746	0.722
	0.777
2ng/ml	0.404	0.357	0.333
	0.31
1ng/ml	0.151	0.182	0.157
	0.212
0ng/ml	0.017	0.024	0
	0.031

We measured random samples of E7195Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	7.5	0.3	3.4
Intra-assay	2	18	4.8	0.2	4.2
Intra-assay	3	18	3	0.2	5.4

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