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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E4060Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/ml	2.048	2.12	2.062
	2.192
200ng/ml	1.795	1.766	1.709
	1.738
100ng/ml	1.233	1.214	1.157
	1.195
50ng/ml	0.757	0.742	0.684
	0.727
25ng/ml	0.397	0.375	0.317
	0.352
0ng/ml	0.063	0.057	0
	0.052

We measured random samples of E4060Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	110.1	4.8	4.4
Intra-assay	2	18	68.1	3.7	5.4
Intra-assay	3	18	49.4	2.5	5

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