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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1296Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200pg/ml	2.262	2.266	2.219
	2.27
600pg/ml	1.72	1.666	1.619
	1.612
300pg/ml	1.114	1.139	1.092
	1.164
150pg/ml	0.646	0.654	0.607
	0.662
75pg/ml	0.408	0.419	0.372
	0.429
0pg/ml	0.051	0.047	0
	0.042

We measured random samples of E1296Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	177.6	11.5	6.5
Intra-assay	2	18	477.8	21.1	4.4
Intra-assay	3	18	93.8	7	7.5

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