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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1900Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400nmol/L	1.704	1.798	1.752
	1.892
1200nmol/L	0.917	0.899	0.853
	0.881
600nmol/L	0.592	0.523	0.477
	0.454
300nmol/L	0.171	0.224	0.178
	0.276
150nmol/L	0.137	0.124	0.078
	0.111
0nmol/L	0.056	0.046	0
	0.036

We measured random samples of E1900Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	739.9	40.8	5.5
Intra-assay	2	18	1009.3	49.7	4.9
Intra-assay	3	18	2227.5	70	3.1

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