Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0208Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ug/ml | 2.309 | 2.172 | 2.13 |
| 2.035 | |||
| 6ug/ml | 1.346 | 1.316 | 1.273 |
| 1.285 | |||
| 3ug/ml | 0.949 | 0.945 | 0.903 |
| 0.941 | |||
| 1.5ug/ml | 0.487 | 0.48 | 0.437 |
| 0.472 | |||
| 0.75ug/ml | 0.238 | 0.233 | 0.19 |
| 0.227 | |||
| 0ug/ml | 0.052 | 0.042 | 0 |
| 0.033 |
Precision
We measured random samples of E0208Ca within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 3.4 | 0.2 | 5.7 |
| Intra-assay | 2 | 18 | 5.5 | 0.2 | 3.3 |
| Intra-assay | 3 | 18 | 2.3 | 0.2 | 7.6 |
Customer reviews and Q&As