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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0010Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ug/ml	2.041	1.972	1.908
	1.903
160ug/ml	1.052	1.083	1.018
	1.113
80ug/ml	0.616	0.633	0.569
	0.651
40ug/ml	0.309	0.316	0.252
	0.324
20ug/ml	0.156	0.151	0.087
	0.146
0ug/ml	0.068	0.064	0
	0.06

We measured random samples of E0010Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	174.3	8.8	5
Intra-assay	2	18	276.6	8.1	2.9
Intra-assay	3	18	55.3	2.4	4.4

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