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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0707Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24mIU/L	2.25	2.187	2.133
	2.124
12mIU/L	1.421	1.416	1.362
	1.412
6mIU/L	0.959	0.913	0.859
	0.867
3mIU/L	0.552	0.548	0.494
	0.545
1.5mIU/L	0.309	0.298	0.243
	0.286
0mIU/L	0.064	0.054	0
	0.045

We measured random samples of E0707Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	12.8	0.4	3.4
Intra-assay	2	18	6.1	0.3	5.1
Intra-assay	3	18	3.6	0.3	8.1

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