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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1441Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400pg/ml	2.073	2.1	2.008
	2.127
1200pg/ml	1.224	1.209	1.117
	1.194
600pg/ml	0.579	0.566	0.474
	0.553
300pg/ml	0.322	0.328	0.236
	0.333
150pg/ml	0.167	0.175	0.083
	0.183
0pg/ml	0.087	0.092	0
	0.097

We measured random samples of E1441Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	704.1	35.4	5
Intra-assay	2	18	2014.7	46	2.3
Intra-assay	3	18	1088.8	45.9	4.2

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