Bovine Malondialdehyde, MDA ELISA Kit

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OneStep ELISA kit
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Home > Product > ELISA Kit > Bovine Malondialdehyde, MDA ELISA Kit

Bovine Malondialdehyde, MDA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.11nmol/ml

Detection range: 0.2-60nmol/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Bovine

Product Summary

Size: 48T,96T

Sensitivity: 0.11nmol/ml

Detection range: 0.2-60nmol/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Bovine

Product overview

Full product name

Malondialdehyde

Code

E0198Bo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.11nmol/ml

Detection range

0.2-60nmol/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Bovine

Storage

2-8°C

Assay time

1h 30m

Background

Malondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors. MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.

Research area

Neuroscience;Signal Transduction;Metabolism

Target protein

MDA

Components

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

Protocol data

These standard curves of E0198Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40nmol/ml	2.366	2.258	2.222
	2.151
20nmol/ml	1.779	1.815	1.779
	1.851
10nmol/ml	1.291	1.248	1.212
	1.206
5nmol/ml	0.749	0.704	0.668
	0.659
2.5nmol/ml	0.42	0.389	0.353
	0.359
0nmol/ml	0.034	0.036	0
	0.039

Precision

We measured random samples of E0198Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	5.7	0.3	5.4
Intra-assay	2	18	15.3	0.9	5.7
Intra-assay	3	18	3.6	0.2	5.3

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Associated products

Datasheet

Size

Code

Price

48T

E0198Bo

$320.00
96T

E0198Bo

$458.00

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Lead time: Within two weeks

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