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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0456Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.24	2.285	2.256
	2.33
12ng/ml	1.303	1.356	1.327
	1.41
6ng/ml	0.671	0.624	0.596
	0.577
3ng/ml	0.304	0.34	0.311
	0.376
1.5ng/ml	0.15	0.171	0.142
	0.192
0ng/ml	0.022	0.029	0
	0.035

We measured random samples of E0456Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	6.1	0.3	4.6
Intra-assay	2	18	11.7	0.3	2.9
Intra-assay	3	18	9.3	0.3	3

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