Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0312Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16ng/ml | 2.272 | 2.154 | 2.108 |
| 2.037 | |||
| 8ng/ml | 1.338 | 1.331 | 1.285 |
| 1.324 | |||
| 4ng/ml | 0.554 | 0.567 | 0.52 |
| 0.579 | |||
| 2ng/ml | 0.335 | 0.329 | 0.282 |
| 0.323 | |||
| 1ng/ml | 0.157 | 0.163 | 0.117 |
| 0.169 | |||
| 0ng/ml | 0.043 | 0.046 | 0 |
| 0.049 |
Precision
We measured random samples of E0312Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 11.6 | 0.4 | 3.2 |
| Intra-assay | 2 | 18 | 3.7 | 0.2 | 4.4 |
| Intra-assay | 3 | 18 | 6.1 | 0.3 | 5 |
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