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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E4358Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.685	2.638	2.596
	2.591
32ng/ml	1.58	1.575	1.533
	1.571
16ng/ml	0.784	0.76	0.717
	0.735
8ng/ml	0.519	0.508	0.465
	0.496
4ng/ml	0.278	0.274	0.231
	0.27
0ng/ml	0.057	0.042	0
	0.028

We measured random samples of E4358Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	9.8	0.5	5.1
Intra-assay	2	18	40.5	1.1	2.6
Intra-assay	3	18	15.6	0.9	5.7

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