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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E3155Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20nmol/L	2.385	2.425	2.376
	2.465
10nmol/L	1.549	1.493	1.444
	1.436
5nmol/L	0.73	0.769	0.72
	0.807
2.5nmol/L	0.448	0.487	0.438
	0.527
1.25nmol/L	0.265	0.235	0.186
	0.205
0nmol/L	0.059	0.049	0
	0.039

We measured random samples of E3155Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	6.1	0.3	5.5
Intra-assay	2	18	9.6	0.3	3.2
Intra-assay	3	18	4.5	0.3	6.6

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