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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1285Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.604	2.482	2.428
	2.359
600ng/L	1.348	1.426	1.372
	1.503
300ng/L	0.86	0.886	0.833
	0.912
150ng/L	0.434	0.452	0.399
	0.471
75ng/L	0.285	0.259	0.206
	0.233
0ng/L	0.05	0.054	0
	0.058

We measured random samples of E1285Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	303.5	16.7	5.5
Intra-assay	2	18	526.9	19.7	3.7
Intra-assay	3	18	120.9	6.9	5.7

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