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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0989Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.255	2.313	2.265
	2.37
400ng/L	1.719	1.821	1.773
	1.923
200ng/L	1.276	1.228	1.18
	1.18
100ng/L	0.688	0.649	0.601
	0.61
50ng/L	0.384	0.405	0.357
	0.426
0ng/L	0.035	0.048	0
	0.061

We measured random samples of E0989Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	179.2	10.7	5.9
Intra-assay	2	18	261.8	10	3.8
Intra-assay	3	18	101.2	5.9	5.8

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