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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0620Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320pg/ml	2.354	2.283	2.223
	2.212
160pg/ml	1.278	1.352	1.291
	1.426
80pg/ml	0.834	0.774	0.713
	0.713
40pg/ml	0.497	0.491	0.43
	0.485
20pg/ml	0.306	0.291	0.231
	0.277
0pg/ml	0.048	0.06	0
	0.072

We measured random samples of E0620Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	48.6	2	4.1
Intra-assay	2	18	81.5	4.2	5.2
Intra-assay	3	18	218.6	7.8	3.6

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