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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0289Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320pg/ml	2.188	2.274	2.243
	2.359
160pg/ml	1.395	1.333	1.301
	1.271
80pg/ml	0.744	0.753	0.722
	0.762
40pg/ml	0.432	0.425	0.393
	0.418
20pg/ml	0.141	0.143	0.112
	0.146
0pg/ml	0.03	0.031	0
	0.032

We measured random samples of E0289Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	200.1	5.3	2.6
Intra-assay	2	18	53.3	3.1	5.7
Intra-assay	3	18	111.3	5.7	5.1

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