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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1593Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.962	1.895	1.843
	1.828
12ng/ml	0.908	0.943	0.891
	0.977
6ng/ml	0.434	0.482	0.43
	0.531
3ng/ml	0.296	0.22	0.168
	0.143
1.5ng/ml	0.087	0.099	0.047
	0.111
0ng/ml	0.059	0.052	0
	0.044

We measured random samples of E1593Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	13	0.6	4.7
Intra-assay	2	18	16.6	0.5	2.8
Intra-assay	3	18	4.8	0.2	4.3

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