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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E6818Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.268	2.318	2.281
	2.367
1000ng/L	1.886	1.817	1.78
	1.748
500ng/L	1.111	1.214	1.177
	1.317
250ng/L	0.604	0.64	0.603
	0.676
125ng/L	0.362	0.375	0.338
	0.389
0ng/L	0.037	0.037	0
	0.037

We measured random samples of E6818Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	621	21.8	3.5
Intra-assay	2	18	204	9.8	4.8
Intra-assay	3	18	303.9	17.9	5.9

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