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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E4142Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.405	2.382	2.334
	2.358
6ng/ml	1.471	1.522	1.475
	1.573
3ng/ml	0.868	0.889	0.842
	0.909
1.5ng/ml	0.38	0.413	0.366
	0.446
0.75ng/ml	0.278	0.243	0.196
	0.209
0ng/ml	0.06	0.047	0
	0.034

We measured random samples of E4142Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	4.5	0.2	4
Intra-assay	2	18	5.9	0.2	2.7
Intra-assay	3	18	1.6	0.1	5

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