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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E6666Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	1.992	1.989	1.967
	1.987
16ng/ml	1.544	1.549	1.527
	1.555
8ng/ml	0.712	0.7	0.677
	0.687
4ng/ml	0.42	0.369	0.347
	0.318
2ng/ml	0.116	0.12	0.097
	0.123
0ng/ml	0.023	0.022	0
	0.021

We measured random samples of E6666Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	18.8	0.9	4.9
Intra-assay	2	18	6.4	0.2	3.4
Intra-assay	3	18	11.6	0.7	5.6

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