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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E4269Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	1.732	1.858	1.804
	1.983
4ng/ml	1.005	0.939	0.886
	0.873
2ng/ml	0.505	0.567	0.513
	0.628
1ng/ml	0.208	0.23	0.177
	0.252
0.5ng/ml	0.115	0.11	0.056
	0.104
0ng/ml	0.054	0.053	0
	0.052

We measured random samples of E4269Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	3	0.1	3.5
Intra-assay	2	18	6	0.2	2.7
Intra-assay	3	18	3.5	0.2	4.9

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