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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0072Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4ng/ml	2.039	2.062	1.995
	2.086
2ng/ml	1.075	1.107	1.039
	1.139
1ng/ml	0.593	0.605	0.538
	0.618
0.5ng/ml	0.294	0.302	0.234
	0.309
0.25ng/ml	0.172	0.182	0.114
	0.191
0ng/ml	0.071	0.068	0
	0.064

We measured random samples of E0072Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	1.9	0.1	4.4
Intra-assay	2	18	2.8	0.1	2.1
Intra-assay	3	18	1.1	0	4.4

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