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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0216Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/ml	2.131	2.078	1.998
	2.026
160ng/ml	1.186	1.148	1.068
	1.11
80ng/ml	0.589	0.608	0.528
	0.627
40ng/ml	0.306	0.319	0.239
	0.332
20ng/ml	0.164	0.153	0.073
	0.142
0ng/ml	0.087	0.08	0
	0.073

We measured random samples of E0216Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	107.2	5.1	4.7
Intra-assay	2	18	207.6	4.1	2
Intra-assay	3	18	83.1	3.9	4.6

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