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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0785Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64umol/L	2.115	2.083	2
	2.051
32umol/L	1.175	1.142	1.06
	1.11
16umol/L	0.581	0.602	0.519
	0.623
8umol/L	0.308	0.32	0.237
	0.332
4umol/L	0.179	0.185	0.103
	0.192
0umol/L	0.081	0.083	0
	0.085

We measured random samples of E0785Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	36.2	1.7	4.7
Intra-assay	2	18	50.2	1.6	3.1
Intra-assay	3	18	10.9	0.6	5.2

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