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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1414Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	2.148	2.06	2.024
	1.972
2000ng/L	1.169	1.079	1.043
	0.988
1000ng/L	0.565	0.574	0.538
	0.582
500ng/L	0.266	0.273	0.237
	0.28
250ng/L	0.121	0.122	0.085
	0.122
0ng/L	0.046	0.036	0
	0.026

We measured random samples of E1414Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	2683	69.9	2.6
Intra-assay	2	18	1321.9	70.9	5.4
Intra-assay	3	18	1695.3	66.3	3.9

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