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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0612Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240nmol/ml	2.094	2.156	2.109
	2.217
120nmol/ml	1.949	1.935	1.888
	1.921
60nmol/ml	1.131	1.129	1.082
	1.127
30nmol/ml	0.686	0.71	0.663
	0.734
15nmol/ml	0.401	0.411	0.364
	0.42
0nmol/ml	0.058	0.047	0
	0.036

We measured random samples of E0612Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	108.6	7.5	6.9
Intra-assay	2	18	16.4	0.8	4.9
Intra-assay	3	18	38.3	1.8	4.8

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