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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0026Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
96ng/L	2.287	2.199	2.168
	2.111
48ng/L	1.326	1.322	1.29
	1.318
24ng/L	0.7	0.626	0.595
	0.553
12ng/L	0.349	0.354	0.322
	0.359
6ng/L	0.132	0.157	0.126
	0.182
0ng/L	0.031	0.032	0
	0.032

We measured random samples of E0026Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	69.7	3	4.4
Intra-assay	2	18	23.1	1	4.2
Intra-assay	3	18	40.8	1.6	3.9

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