Tel:86 021 3100 7137

2024 Holiday Shipping Notification: Korain Biotech will be closed from 3rd to 17th February 2024
Home > Product > ELISA Kit > Human Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Human Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Human Insulin-like Growth Factors 1, IGF-1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.058ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.058ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Insulin-like Growth Factors 1

Code

E0103Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.058ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

The Insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed: 21076856, PubMed: 24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed: 19578119, PubMed: 22351760, PubMed: 23696648, PubMed: 23243309).

UniProt accession

MASS(Da)

21,841

GeneID

3479

Synonyms

IGF 1;IGF1;IGF-I;Insulin-like growth factor I;Mechano growth factor;MGF;Somatomedin-C

Gene names

IGF1

Research area

Signal Transduction; Cancer; Developmental Biology

Target protein

IGF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0103Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.247 2.19 2.132
2.133
12ng/ml 1.594 1.627 1.569
1.66
6ng/ml 1.023 1.077 1.018
1.13
3ng/ml 0.701 0.674 0.615
0.646
1.5ng/ml 0.443 0.429 0.371
0.415
0ng/ml 0.059 0.058 0
0.058

Precision

We measured random samples of E0103Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.5 0.43 5.1
Intra-Assay 2 18 6.9 0.51 7.4
Intra-Assay 3 18 2.2 0.15 6.7

Protocols

References (3)

Impact of maternal dietary carbohydrate intake and vitamin D-related genetic risk score on birth length: the Vitamin D Pregnant Mother (VDPM) cohort study
Evaluation of the relationship between pain inflammation due to dental caries and growth parameters in preschool children
Evaluation of circulating insulin-like growth factor-1, heart-type fatty acid-binding protein, and endotrophin levels as prognostic markers of COVID-19 infection severity

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 96T

    E0103Hu

    $458.00

  • 48T

    E0103Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up