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Home > Product > ELISA Kit > Mouse Interferon Regulatory Factor 4, IRF4 ELISA Kit

Mouse Interferon Regulatory Factor 4, IRF4 ELISA Kit

Mouse Interferon Regulatory Factor 4, IRF4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.34ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.34ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interferon Regulatory Factor 4

Code

E2162Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.34ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.

UniProt accession

MASS(Da)

51,577

GeneID

16364

Synonyms

Interferon regulatory factor 4;IRF 4;IRF4;IRF-4;LSIRF;Lymphocyte-specific interferon regulatory factor;NF-EM5;PU.1 interaction partner;Transcriptional activator PIP;

Gene names

IRF4

Research area

Others

Target protein

IRF4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2162Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.086 2.049 1.995
2.012
600ng/L 1.311 1.402 1.348
1.493
300ng/L 0.778 0.775 0.72
0.772
150ng/L 0.536 0.49 0.436
0.444
75ng/L 0.298 0.3 0.246
0.303
0ng/L 0.048 0.055 0
0.061

Precision

We measured random samples of E2162Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 225.1 12.28 5.5
Intra-Assay 2 18 266.8 16.18 6.1
Intra-Assay 3 18 700.3 28.2 4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2162Mo

    $320.00

  • 96T

    E2162Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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