Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Mouse Long-chain-Fatty-acid--CoA Ligase 1, ACSL1 ELISA Kit

Mouse Long-chain-Fatty-acid--CoA Ligase 1, ACSL1 ELISA Kit

Mouse Long-chain-Fatty-acid--CoA Ligase 1, ACSL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.02ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.02ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Long-Chain-Fatty-Acid--Coa Ligase 1

Code

E2218Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.02ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (By similarity). Preferentially uses palmitoleate, oleate and linoleate (By similarity). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs).

UniProt accession

MASS(Da)

77,951

GeneID

14081

Synonyms

ACSL 1;ACSL1;Arachidonate--CoA ligase;LACS 1;Long-chain acyl-CoA synthetase 1;Long-chain-fatty-acid--CoA ligase 1;Phytanate--CoA ligase

Gene names

ACSL1

Research area

Signal Transduction

Target protein

ACSL1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2218Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.497 2.479 2.442
2.46
1000ng/L 1.483 1.445 1.408
1.406
500ng/L 0.774 0.831 0.794
0.888
250ng/L 0.45 0.414 0.377
0.377
125ng/L 0.203 0.225 0.188
0.247
0ng/L 0.043 0.037 0
0.031

Precision

We measured random samples of E2218Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 618.8 31.46 5.1
Intra-Assay 2 18 1056.8 29.54 2.8
Intra-Assay 3 18 341.3 17.86 5.2

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E2218Mo

    $320.00

  • 96T

    E2218Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up