Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Mouse R-spondin-2, RSPO2 ELISA Kit

Mouse R-spondin-2, RSPO2 ELISA Kit

Mouse R-spondin-2, RSPO2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.95ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.95ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

R-Spondin-2

Code

E2435Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.95ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors (PubMed: 21693646). During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form (By similarity).

UniProt accession

MASS(Da)

28,276

GeneID

239405

Synonyms

Cristin-2;Cysteine-rich and single thrombospondin domain-containing protein 2;mCristin-2;Roof plate-specific spondin-2;RSPO 2;RSPO2;R-spondin-2

Gene names

RSPO2

Research area

Developmental Biology

Target protein

RSPO2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2435Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.003 1.916 1.873
1.83
1200ng/L 1.126 1.005 0.961
0.883
600ng/L 0.618 0.541 0.497
0.463
300ng/L 0.288 0.286 0.243
0.285
150ng/L 0.135 0.132 0.088
0.128
0ng/L 0.053 0.043 0
0.033

Precision

We measured random samples of E2435Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 655.4 31.03 4.7
Intra-Assay 2 18 2007.4 49.85 2.5
Intra-Assay 3 18 988.2 31.62 3.2

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E2435Mo

    $320.00

  • 96T

    E2435Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up