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Home > Product > ELISA Kit > Mouse Triosephosphate Isomerase, TPI1 ELISA Kit

Mouse Triosephosphate Isomerase, TPI1 ELISA Kit

Mouse Triosephosphate Isomerase, TPI1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.58ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.58ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Triosephosphate Isomerase

Code

E2526Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.58ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis. It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.

UniProt accession

MASS(Da)

26,713

GeneID

21991

Synonyms

Methylglyoxal Synthase;TIM;TPI 1;TPI1;Triosephosphate isomerase;Triose-phosphate isomerase

Gene names

TPI1

Research area

Others

Target protein

TPI1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2526Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.227 2.285 2.231
2.343
240ng/L 1.925 1.887 1.833
1.849
120ng/L 1.098 1.205 1.15
1.312
60ng/L 0.679 0.681 0.627
0.683
30ng/L 0.361 0.387 0.333
0.414
0ng/L 0.049 0.054 0
0.06

Precision

We measured random samples of E2526Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 101.7 5.4 5.3
Intra-Assay 2 18 166.7 6.78 4.1
Intra-Assay 3 18 30.4 1.65 5.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2526Mo

    $320.00

  • 96T

    E2526Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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