Mouse Peroxisome Proliferation Activated Receptor Gamma, PPARG ELISA Kit

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Home > Product > ELISA Kit > Mouse Peroxisome Proliferation Activated Receptor Gamma, PPARG ELISA Kit

Mouse Peroxisome Proliferation Activated Receptor Gamma, PPARG ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.012ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.012ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Peroxisome Proliferation Activated Receptor Gamma

Code

E2630Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.012ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (PubMed: 19041764).

UniProt accession

P37238

MASS（Da）

57,598

GeneID

19016

Synonyms

Nuclear receptor subfamily 1 group C member 3;Peroxisome proliferator-activated receptor gamma;PPAR G;PPARG;PPAR-gamma

Gene names

PPARG

Research area

Metabolism

Target protein

PPARG

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E2630Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.009	1.989	1.963
	1.968
6ng/ml	1.492	1.455	1.429
	1.418
3ng/ml	0.586	0.596	0.57
	0.606
1.5ng/ml	0.415	0.395	0.369
	0.375
0.75ng/ml	0.202	0.195	0.17
	0.189
0ng/ml	0.024	0.026	0
	0.028

Precision

We measured random samples of E2630Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.6	0.12	4.5
Intra-Assay	2	18	8.9	0.44	5
Intra-Assay	3	18	4.3	0.2	4.6

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E2630Mo

$320.00
96T

E2630Mo

$458.00

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Lead time: Within two weeks

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