Human Interleukin 1Β, IL-1B ELISA Kit

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Home > Product > ELISA Kit > Human Interleukin 1Β, IL-1B ELISA Kit

Human Interleukin 1Β, IL-1B ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 10.07pg/mL

Detection range: 20-6000pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 10.07pg/mL

Detection range: 20-6000pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin 1 beta

Code

E0143Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

10.07pg/mL

Detection range

20-6000pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed: 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed: 12794819).

UniProt accession

P01584

MASS（Da）

30,748

GeneID

3553

Synonyms

Catabolin;IL 1B;IL 1beta;IL-1 beta;IL1B;Interleukin-1 beta

Gene names

IL1B

Research area

Immunology; Microbiology; Cardiovascular; Metabolism

Target protein

IL1B

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0143Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000pg/mL	2.364	2.272	2.23
	2.18
2000pg/mL	1.378	1.317	1.275
	1.256
1000pg/mL	0.807	0.724	0.682
	0.642
500pg/mL	0.443	0.463	0.421
	0.483
250pg/mL	0.195	0.208	0.166
	0.222
0pg/mL	0.027	0.042	0
	0.058

Precision

We measured random samples of E0143Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1899.9	61.65	3.2
Intra-Assay	2	18	821.3	40.18	4.9
Intra-Assay	3	18	1765.1	88.88	5

Protocols

> Protocol Booklet

References (12)

Effect of high amylose resistant starch (HAM‐RS2) supplementation on biomarkers of inflammation and oxidative stress in hemodialysis patients: a randomized clinical trial

Journal: Hemodial Int. 2018 Oct;22(4):492-500.

The Relationship between Serum Concentrations of Pro- and Anti-Inflammatory Cytokines and Nutritional Status in Patients with Traumatic Head Injury in the Intensive Care Unit

Journal: Medicina (Kaunas). 2019 Aug 15;55(8):486.

Different spexin level in obese vs normal weight children and its relationship with obesity related risk factors

Journal: Nutr Metab Cardiovasc Dis. 2020 Apr 12;30(4):674-682

Correlation between serum pro inflammatory cytokines and clinical scores of knee osteoarthritic patients using resveratrol as a supplementary therapy with meloxicam

Journal: Indian J Pharmacol. 2021 Jul-Aug;53(4):270-277.

The Potential Predictive Role of Tumour Necrosis Factor-α, Interleukin-1β, and Monocyte Chemoattractant Protein-1 for COVID-19 Patients Survival

Journal: Infect Drug Resist. 2022 Mar 4;15:821-829.

View all publications for the product

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Associated products

Datasheet

Size

Code

Price

96T

E0143Hu

$458.00
48T

E0143Hu

$320.00

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Lead time: Within two weeks

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