Full product name

Code

Assay type

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Detection range

Sample type

Species

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Assay time

Gene names

Research area

Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1446Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24umol/ml	2.137	2.149	2.077
	2.162
12umol/ml	1.075	1.111	1.038
	1.146
6umol/ml	0.619	0.646	0.573
	0.673
3umol/ml	0.292	0.282	0.209
	0.272
1.5umol/ml	0.16	0.151	0.079
	0.142
0umol/ml	0.068	0.073	0
	0.077

We measured random samples of E1446Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	16.7	0.4	2.4
Intra-assay	2	18	11.9	0.5	4.2
Intra-assay	3	18	4	0.2	3.8

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