Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Storage
Assay time
UniProt accession
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E7970Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 2.014 | 2.054 | 1.986 |
| 2.095 | |||
| 6ng/ml | 1.215 | 1.236 | 1.168 |
| 1.258 | |||
| 3ng/ml | 0.615 | 0.592 | 0.524 |
| 0.568 | |||
| 1.5ng/ml | 0.29 | 0.306 | 0.237 |
| 0.322 | |||
| 0.75ng/ml | 0.162 | 0.172 | 0.104 |
| 0.182 | |||
| 0ng/ml | 0.071 | 0.068 | 0 |
| 0.065 |
Precision
We measured random samples of E7970Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 3.2 | 0.2 | 5.4 |
| Intra-assay | 2 | 18 | 3.9 | 0.2 | 4.2 |
| Intra-assay | 3 | 18 | 7.8 | 0.2 | 2.8 |
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